Jahr Name
2022

Hochleistungs-Lateral Flow Assays durch zentrifugalmikrofluidische Automatisierung (CentrImA)

B. Breiner, D. Kainz

Abschlussbericht IGF-Vorhaben Nr. 20436 N

2022

Paper-based open microfluidic platform forprotein electrophoresis and immunoprobing

S. Hennig, Z. Shu, L. Gutzweiler, P. Koltay, F. von Stetten, R. Zengerle, S. M. Früh

Electrophoresis, 43 (4), 621-631, doi: 10.1002/elps.202100327
 

Kurzfassung einblenden

Protein electrophoresis and immunoblotting are indispensable analytical tools for the characterization of proteins and posttranslational modifications in complex sample matrices. Owing to the lack of automation, commonly employed slab-gel systems suffer from high time demand, significant sample/antibody consumption, and limited reproducibility. To overcome these limitations, we developed a paper-based open microfluidic platform for electrophoretic protein separation and subsequent transfer to protein-binding membranes for immunoprobing. Electrophoresis microstructures were digitally printed into cellulose acetate membranes that provide mechanical stability while maintaining full accessibility of the microstructures for consecutive immunological analysis. As a proof-of-concept, we demonstrate separation of fluorescently labeled marker proteins in a wide molecular weight range (15–120 kDa) within only 15 min, reducing the time demand for the entire workflow (from sample preparation to immunoassay) to approximately one hour. Sample consumption was reduced 10- to 150-fold compared to slab-gel systems, owing to system miniaturization. Moreover, we successfully applied the paper-based approach to complex samples such as crude bacterial cell extracts. We envisage that this platform will find its use in protein analysis workflows for scarce and precious samples, providing a unique opportunity to extract profound immunological information from limited sample amounts in a fast fashion with minimal hands-on time.

 

Link to publication

2022

Proteinelektrophorese in Offener Mikrofluidik (PRINTOMik)

A. Klebes

Abschlussbericht IGF-Vorhaben Nr. 20489 N
 

2022

Methods— A Simple Method to Measure In-Plane Electrical Resistance of PEM Fuel Cell and Electrolyzer Catalyst Layers

L. Bohn, M. von Holst, E. Cruz Ortiz, M. Breitwieser, S. Vierrath, C. Klose

J. Electrochem. Soc., 169 (5), 054518, doi: 10.1149/1945-7111/ac6e09

2022

Mechanical properties of polycaprolactone (PCL) scaffolds for hybrid 3D-bioprinting with alginate-gelatin hydrogel

F. Koch, O. Thaden, S. Conrad, K. Tröndle, G. Finkenzeller, R. Zengerle, S. Kartmann, S. Zimmermann, P. Koltay

 J. Mech. Behav. Biomed. Mat., 130, 105219, doi: 10.1016/j.jmbbm.2022.105219

2022

Deep Learning-Assisted Nephrotoxicity Testing with Bioprinted Renal Spheroids

K. Tröndle, G. Miotto, L. Rizzo, R. Pichler, F. Koch, P. Koltay, R. Zengerle, S. S. Lienkamp, S. Kartmann, S. Zimmermann

Deep Learning-Assisted Nephrotoxicity Testing with Bioprinted Renal Spheroids

2022

ImmunoDisk—A Fully Automated Bead-Based Immunoassay Cartridge with All Reagents Pre-Stored

B. Johannsen, D. Baumgartner, L. Karkossa, N. Paust, M.l Karpíšek, N. Bostanci, R. Zengerle, K. Mitsakakis

Biosensors 12 (6), 413, doi: 10.3390/bios12060413

2022

Microfluidic One-Pot Digital Droplet FISH Using LNA/DNA Molecular Beacons for Bacteria Detection and Absolute Quantification

Y.-T. Kao, S. Calabrese, N. Borst, M. Lehnert, Y.-K. Lai, F. Schlenker, P. Juelg, R. Zengerle, P. Garstecki, F. von Stetten

Biosensors 2022, 12(4), 237; doi: 10.3390/bios12040237

2021

Die MRD Disk: Automatisiertes Monitoring Minimaler Resterkrankung durch hochsensitive, zentrifugal-mikrofluidische Multiplex-qPCR / The MRD disk: automated minimal residual disease monitoring by highly sensitive centrifugal microfluidic multiplex qPCR

P. Juelg, E. Kipf, M. Specht, J. Menges, M. Fillies, C. Eckert, N. Paust, R. Zengerle, M. Lehnert, T. Hutzenlaub

MST-Kongress, Ludwigsburg, 08.-10.11.2021

2021

High dynamic range digital assay enabled by dual volume centrifugal step emulsification

M. Schulz, J. Ruediger, E. Landmann, M. Bakheit, S. Frischmann, D. Rassler, A. R. Homann, F. von Stetten, R. Zengerle, N.Paust

MST-Kongress, Ludwigsburg, 08.-10.11.2021

2021

Hybrider 3D-Biodruck zur künstlichen Herstellung von Knochen / Using hybrid processes for 3D-bioprinting of artificial bone tissue

F. Koch, K. Tröndle, G. Finkenzeller, P. Rukavina, R. Zengerle, P. Koltay, S. Zimmermann

MST-Kongress, Ludwigsburg, 08.-10.11.2021

2021

Integration of nanoporous gold electrodes in microfluidic chambers by inkjet-printing for biosensing applications

M. Trotter, D. Juric, Z. Bagherian, N. Borst, K. Gläser, F. von Stetten, A. Zimmermann

MST-Kongress, Ludwigsburg, 08.-10.11.2021

2021

Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP

L. Becherer, J.F. Hess, S. Frischmann, M. Bakheit, H. Nitschko, S. Stinco, F. Zitz, H. Hofer, G. Porro, F. Hausladen, K. Stock, D. Drossart, H. Wurm, H. Kuhn, D. Huber, T. Hutzenlaub, N. Paust, M. Keller, O. Strohmeier, S. Wadle, N. Borst, R. Zengerle, F. von Stetten

MST-Kongress, Ludwigsburg, 08.-10.11.2021

2021

Quantitative STEM Tomography For Proton Exchange Membrane Fuel Cell Applications

P. A. Heizmann, C. Klose, M. Breitwieser, S. Vierrath

Nano-BW 2021, Bad Herrenalb, Germany, online, 7.-8.10.2021

2021

Drop-on-Demand Bioprinting Approach For Precise Alignment and Interaction Studies of Different Cell Types

J. Weygant, F. Koch, K. Troendle, G. Finkenzeller, R. Zengerle, S. Kartmann, S. Zimmermann, P. Koltay

International Conference on Biofabrication, Australia, 27. – 29.09.2021, online

2021

Generation of platelet poor plasma for circulating cell-free mitochondrial DNA analysis on a centrifugal microfluidic platform

F. Schlenker, L. Karkossa, N. Paust, R. Zengerle, S. Giaglis, U. A. Walker, T. Hutzenlaub, P. Juelg

Micro and Nano Engineering Conference, Turin, Italy, 20. – 23.09.2021

2021

Large sample volume management on centrifugal microfluidic cartridges

J. Schlanderer, J. Lüddecke, R. Zengerle, N. Paust

Micro and Nano Engineering Conference, Turin, Italy, 20. – 23.09.2021

2021

Waste liquid assisted flow-switching on a centrifugal microfluidic platform

E. Mahmodi Arjmand, G. Grether, R. Zengerle, N. Paust, J. Lüddecke

Micro and Nano Engineering Conference, Turin, Italy, 20. – 23.09.2021

2021

Universal multiplex mediator displacement LAMP Nr. P3.062 analytical and clinical validation on the example of HIV, Haemophilus ducreyi and Treponema pallidum

T. Härpfer, L. Becherer, M. Bakheit, S. Frischmann, S. Stinco, N. Borst, R. Zengerle, F. von Stetten

Biosensors, , Haeundae-gu, South Korea, online, 26. – 29.07.2021

2021

SuperPests LabDisk: a microfluidic-based molecular diagnostic platform for detection of biotypes, resistance mutations and plant pathogens P3.021

D. Baumgartner, B. Johannsen, N. Paust, F. von Stetten, R. Zengerle, K. Mavridis, J. Vontas, K. Mitsakakis

Biosensors, Haeundae-gu, South Korea, online, 26. – 29.07.2021