Jahr Name
2022

PEM fuel cells based on novel hydrocarbon ionomers: Approaching state-of-the-art performance

H. Nguyen, D. Sultanova, F. Lombeck, C. Schwarz, P. A. Heizmann, C. Klose, S. Vierrath, M. Breitwieser

Gordon Research Conference, July 24 - 29, 2022, Smithfield, RI, USA    

2022

Microthermoforming: A low cost process for automated small-scale production

P. Koch, O. Barth, M. Jehle, R. Streller, R. Zengerle, M. Rombach

Symposium Verbundwerkstoffe, July, 20-22, 2022, Leoben & online    

2022

Assessing the electronic and ionic conductivity of NiFe-LDH-based catalyst layers for AEMWEs

M. Elshamy, L. Metzler, S. Vierrath

Workshop on Ion Exchange Membranes for Energy Applications, June 20 – 22, 2022, Bad Zwischenahn     

2022

Fully casted catalyst coated membranes for anion exchange membrane water electrolysis

L. Metzler, S. Koch, M. Elshamy, S. Kilian, M. Breitwieser, S. Vierrath

Workshop on Ion Exchange Membranes for Energy Applications, June 20 – 22, 2022, Bad Zwischenahn     

2022

Influence of the gas diffusion layer on the performance of a PEM water electrolyzer

N. van Treel, E. Cruz Ortiz, S. Vierrath, M. Bühler

ICE 2021, 3rd International Conference on Electrolysis 2021, June 20-23, 2022, Golden, Colorado, USA    

2022

Enabling the large scale production of fluorine free electrode assemblies for proton exchange membrane water electrolysis

C. Schare, A. Münchinger, R. Qelibari, L. Metzler, G. Titvinidze, S. Vierrath, C. Klose

ICE 2021, 3rd International Conference on Electrolysis 2021, June 20-23, 2022, Golden, Colorado, USA    

2022

Water management in anion-exchange membrane water electrolyzers under dry cathode operation

S. Koch, S. K. Kilian, J. L. Disch, L. Metzler, P. A. Heizmann, Y. Han, M. Schulz, M. Breitwieser, S. Vierrath

ICE 2021, 3rd International Conference on Electrolysis 2021, June 20-23, 2022, Golden, Colorado, USA     

2022

Effect of compression on the membrane electrode assembly of polymer electrolyte electrolyzers

E. Cruz Ortiz, M. Breitwieser, S. Vierrath , M. Bühler

ICE 2021, 3rd International Conference on Electrolysis 2021, June 20-23, 2022, Golden, Colorado, USA     

2022

Towards standardization of pre-analytics: Highly efficient platelet-poor-plasma generation for ccfmtDNA analysis on a centrifugal microfluidic platform

T.-Tu Truong, F. Schlenker, L. Karkossa, E. M. Arjmand, G. Grether, J. Lüddecke, U. A. Walker, S. Giaglis, N. Paust, T. Hutzenlaub, P. Juelg

EACR Liquid Biopsy, May 24-26, 2022, Bergamo, Italy    

2022

Paper-based open microfluidics platform for automatic protein analysis

S. Hennig, Z. Shu, L. Gutzweiler, P. Koltay, F. von Stetten, R. Zengerle, S. M. Früh

SLAS Europe 2022 Conference and Exhibition, May 26, 2022, Dublin, Ireland    

2022

A Bioprinting Fidelity Imager (BioFI) for a standardized characterization of bioprinting processes

L. Riek, F. Koch, D. Frejek, L. Zausch, R. Zengerle, P. Koltay, S. Kartmann, S. Zimmermann

SLAS Europe 2022 Conference and Exhibition, May 26, 2022, Dublin, Ireland    

2022

Selective high-throughput deposition of microtumors for in vitro studies on 3D tumor models

V. Zieger, D. Frejek, L. Riek, R. Zengerle, S. Kartmann

SLAS Europe 2022 Conference and Exhibition, May 26, 2022, Dublin, Ireland    

2022

High-resolution neutron imaging of carbonate precipitation and water transport in CO2 electrolysis to CO

J. Disch, L. Bohn, S. Koch, M. Schulz, Y. Han, M. Breitwieser, S. Vierrath

Gordons Solar Fuels, May 8-13, 2022, Lucca, Italy    

2022

Cl doped Ag as highly porous nanoparticles for CO2 electrolysis to CO

K. Seteiz, J. Disch, S. Vierrath

Gordons Solar Fuels, May 8-13, 2022, Lucca, Italy

2022

High-resolution neutron imaging of carbonate precipitation and water transport in CO2 electrolysis to CO

J. Disch, L. Bohn, S. Koch, M. Schulz, Y. Han, M. Breitwieser, S. Vierrath

Swiss Electrochemistry Symposium, May 11,  2022, Aarau, Switzerland

2022

Validation of scRNA-seq fold changes by single-cell reverse transcription digital PCR

T. Lange, T. Groß, Á. Jeney, J. Scherzinger, E. S., C. Niemöller, S. Zimmermann, P. Koltay, F. von Stetten, R. Zengerle, C. Jeney

Single-Cell Genomics Gordon Research Conference, May 1- 6, 2022, Les Diablerets, VD, Switzerland

2022

Microthermoforming - enhancing Blister technology

D. Kainz

12th European Thermoforming Conference 2022, 31st March - 1st April 2022, Vienna, Austria

2022

Hochleistungs-Lateral Flow Assays durch zentrifugalmikrofluidische Automatisierung (CentrImA)

B. Breiner, D. Kainz

Abschlussbericht IGF-Vorhaben Nr. 20436 N

2022

Paper-based open microfluidic platform forprotein electrophoresis and immunoprobing

S. Hennig, Z. Shu, L. Gutzweiler, P. Koltay, F. von Stetten, R. Zengerle, S. M. Früh

Electrophoresis, 43 (4), 621-631, doi: 10.1002/elps.202100327
 

Kurzfassung einblenden

Protein electrophoresis and immunoblotting are indispensable analytical tools for the characterization of proteins and posttranslational modifications in complex sample matrices. Owing to the lack of automation, commonly employed slab-gel systems suffer from high time demand, significant sample/antibody consumption, and limited reproducibility. To overcome these limitations, we developed a paper-based open microfluidic platform for electrophoretic protein separation and subsequent transfer to protein-binding membranes for immunoprobing. Electrophoresis microstructures were digitally printed into cellulose acetate membranes that provide mechanical stability while maintaining full accessibility of the microstructures for consecutive immunological analysis. As a proof-of-concept, we demonstrate separation of fluorescently labeled marker proteins in a wide molecular weight range (15–120 kDa) within only 15 min, reducing the time demand for the entire workflow (from sample preparation to immunoassay) to approximately one hour. Sample consumption was reduced 10- to 150-fold compared to slab-gel systems, owing to system miniaturization. Moreover, we successfully applied the paper-based approach to complex samples such as crude bacterial cell extracts. We envisage that this platform will find its use in protein analysis workflows for scarce and precious samples, providing a unique opportunity to extract profound immunological information from limited sample amounts in a fast fashion with minimal hands-on time.

 

Link to publication

2022

Proteinelektrophorese in Offener Mikrofluidik (PRINTOMik)

A. Klebes

Abschlussbericht IGF-Vorhaben Nr. 20489 N