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Sequencing in wastewater treatment plants to reduce greenhouse gas emissions (SmART)

The Anammox process reduces oxygen demand, sludge and nitrous oxide emissions. The SmART project uses MinION sequencing to efficiently monitor microbiological communities in wastewater treatment plants on site. The automated workflow from sample preparation to analysis enables rapid action in the event of malfunctions.

The anammox process (anaerobic ammonium oxidation) is a wastewater treatment process. Compared to conventional nitrification/denitrification, it has significant advantages such as a reduction in oxygen demand, no carbon requirement for denitrification, a substantial reduction in sludge production and the avoidance of emissions of climate-damaging and ozone-depleting nitrous oxide. However, the process requires precise monitoring of the bacteria involved. The SmART project uses Oxford Nanopore Technologies' MinION sequencing technology to analyze microbial communities in wastewater treatment plants. By automating the entire workflow, from sample preparation to sequencing and bioinformatic analysis, efficient monitoring is made possible. The system thus facilitates the direct integration of biological samples into the on-site analysis process and enables rapid action to be taken in the event of process disruptions.

Project
Sequenzierung vor Ort in Abwasseraufbereitungsanlagen zur Reduzierung von Treibhausgasemissionen (SmART)
Sponsor
investBW
Promoter
VDI/VDE Innovation + Technik GmbH
Funding Number
BW7_1095/02
Duration
01.11.2024
Cooperation Partner
E+H BioSense GmbH, HS Analysis GmbH, Zymo Research Europe GmbH
Maturity Level
Functional model
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Competences

  • Mikrofluidics
  • Lab-on-a-Chip
  • Simulation-based Design