Enzymatic fragmentation and peptide spectrometry through nanopores (ProNanoPep)
The goal of the project is to develop a new approach that combines highly controlled and automated enzymatic fragmentation with a new principle, peptide spectrometry through nanopores, for label-free identification, discrimination and ultimately sequencing of proteins.
The goal is to explore a novel platform for label-free protein identification, discrimination and ultimately sequencing. The platform consists of:
- A microfluidic approach for automated enzymatic fragmentation of polypeptides.
- A biohybrid sensor consisting of a protein nanopore inserted into a biomimetic phospholipid membrane. An array of such nanopore devices is provided directly on a CMOS-based, low-noise current amplifier array.
- Protein-Nanoporen-vermittelte Einzelmoleküldetektion von Peptidfragmenten für Proteindiskrimination und Sequenzierung (ProNanoPep)
Project Management Jülich
- Funding Number
- 01.01.2022 to 31.12.2024
- Cooperation Partner
- Albert-Ludwigs-Universität Freiburg, Ionera Technologies GmbH
- Maturity Level