The goal of project dINA is the development of a platform for droplet based high sensitive protein analytics for the quantification of drug agents and biomarkers to be used in the field of drug development. As part of this a centrifugal microfluidic chip will be designed and manufactured using injection molding at Hahn-Schickard.
Precise quantification of drug agents and biomarkers as well as the detection of substances leading to unspecific immune responses is very important in the field of drug development. As a standard method for quantification ELISA (enzyme-linked immunosorbent assay) is commonly used. Using this technology it can be a challenge to reach high levels of analytical sensitivity as well as a high dynamic range.
Instead of using ELISA, the goal in the project dINA is the quantification of proteins by a newly developed digital Immuno- loop-mediated isothermal amplification (LAMP) (Chimera Biotec GmbH, Mast Diagnostica GmbH). This allows a precise quantification with high sensitivity and high dynamic range across 105 magnitudes.
For digitalization and automation of the immono-LAMP assay a centrifugal-microfluidic chip for the generation of thousands of reaction compartments (droplets) will be designed and manufactured using injection molding at Hahn-Schickard. To complete the platform an automated processing device will be developed by the partner Biofluidix GmbH.back to overview
|Promoter:||Project Management Jülich|
|Duration:||01.07.2017 to 30.06.2020|
|Cooperation Partner:||Chimera Biotec GmbH, Mast Diagnostica GmbH, BioFluidix GmbH|
|Maturity Level:||Research > Research pattern|